HOECHST 33342/PI Fluorescent Stain for Maximum Precision in Viability Determination This product combines the fluorescent stains Hoechst 33342 and Propidium Iodide (PI) for clear differentiation between all nucleated cells and those with damaged cell membranes. Hoechst 33342 is a cell-permeable stain that binds to DNA and stains the nuclei of all cells, while PI does not penetrate the membrane and only stains cells with membrane damage, typically non-viable or in advanced stages of cell death.

Main Benefits

Hoechst 33342 stains the nuclei of all cells, allowing for total cell counting.
PI stains cells with damaged membranes, enabling identification of non-viable cells.
Dual staining allows for simultaneous assessment of total cells versus non-viable cells.
Suitable for fluorescence imaging and flow cytometry analysis.

Composition

Hoechst 33342 Stain (cell-permeable stain that binds to DNA)
Propidium Iodide (PI) Stain (non-permeable stain that intercalates with DNA)

Instructions for Use

Used through co-staining with Hoechst 33342 and PI; add to cell samples in an appropriate medium or culture medium. Apply directly to cell suspensions or cultures. Optimal concentrations and incubation conditions should be determined by a qualified veterinarian or researcher based on sample type and experimental context.

Package Size

Kit format containing separate vials for Hoechst 33342 and PI stains, sufficient for multiple experiments (exact quantity or number of reactions depends on the supplier).

Note

For research use under professional or veterinary supervision. Not intended for diagnostic or therapeutic use.