HOECHST 33342/FITC-PNA FLUORESCENT STAIN ACROSOME INTEGRITY
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HOECHST 33342/FITC‑PNA Fluorescent Stain for Acrosome Integrity Assessment
This veterinary product combines two fluorescent stains—Hoechst 33342 and FITC‑PNA—to assess the acrosome integrity of sperm cells. Hoechst 33342 acts as a blue nuclear stain, facilitating clear identification of sperm cells, while FITC‑PNA (conjugated with fluorescein isothiocyanate) binds to the acrosomal membrane, highlighting the acrosome status under a fluorescent microscope or flow cytometer.
Enables simultaneous visualization of nuclei and acrosome integrity
Allows differentiation between intact and damaged acrosomes based on fluorescence patterns
Applicable to multiple species and compatible with automated systems like AndroVision®
Hoechst 33342 (blue nuclear stain)
FITC‑PNA (green fluorescent lectin targeting the acrosomal membrane)
Intended for use under the supervision of a veterinarian or in professional laboratories
Typically applied to semen samples through staining and analysis by microscopy or flow cytometry
The veterinarian or laboratory specialist should determine the dosage and incubation time based on species, sample type, and clinical context
Designed for up to 100 tests per package
Used in conjunction with systems like AndroVision® for automated acrosome integrity assessment
Numerical dosage tables have been omitted to ensure flexibility and adherence to professional guidance
This veterinary product combines two fluorescent stains—Hoechst 33342 and FITC‑PNA—to assess the acrosome integrity of sperm cells. Hoechst 33342 acts as a blue nuclear stain, facilitating clear identification of sperm cells, while FITC‑PNA (conjugated with fluorescein isothiocyanate) binds to the acrosomal membrane, highlighting the acrosome status under a fluorescent microscope or flow cytometer.
Main Benefits
Enables simultaneous visualization of nuclei and acrosome integrity
Allows differentiation between intact and damaged acrosomes based on fluorescence patterns
Applicable to multiple species and compatible with automated systems like AndroVision®
Composition
Hoechst 33342 (blue nuclear stain)
FITC‑PNA (green fluorescent lectin targeting the acrosomal membrane)
Instructions for Use
Intended for use under the supervision of a veterinarian or in professional laboratories
Typically applied to semen samples through staining and analysis by microscopy or flow cytometry
The veterinarian or laboratory specialist should determine the dosage and incubation time based on species, sample type, and clinical context
Package Size
Designed for up to 100 tests per package
Note
Used in conjunction with systems like AndroVision® for automated acrosome integrity assessment
Numerical dosage tables have been omitted to ensure flexibility and adherence to professional guidance