TL HEPES, Liquid, 50 ml, Medium for Oocytes with Stable pH under Ambient Conditions This veterinary product, TL-HEPES liquid medium in a 50 ml size, is a ready-to-use medium designed for handling and washing oocytes under ambient conditions (not controlled by carbon dioxide). It is based on the well-known Tyrode’s Lactate-HEPES (TL-HEPES) formulation, widely used in reproductive biology procedures to maintain pH stability while handling oocytes outside carbon dioxide-controlled incubators. This medium supports short-term environmental stability and is particularly useful in oocyte collection, washing, and handling procedures before in vitro fertilization or related assisted reproduction techniques.

Main Benefits

Maintains pH stability under ambient conditions (not controlled by carbon dioxide) thanks to the buffering capacity of HEPES
Supports handling and washing of oocytes in reproductive procedures
Ready-to-use liquid formulation in a convenient 50 ml size
Based on the reliable TL-HEPES formulation widely used in veterinary and research settings

Composition

The formulation is based on standard TL-HEPES: includes sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium dihydrogen phosphate, sodium lactate, sodium bicarbonate, HEPES buffer, sodium pyruvate, and bovine serum albumin (BSA) according to published recipes for TL-HEPES medium used in oocyte handling in research and veterinary reproductive contexts. Exact concentrations are not specified here.

Instructions for Use

Used via the liquid route for washing and handling oocytes under ambient conditions. The dosage and volume should be determined by a veterinarian or qualified reproduction specialist based on species, body weight, and clinical condition.

Package Size

One bottle containing 50 ml of TL-HEPES liquid medium.

Note

This product is intended for professional use under veterinary supervision. It is not for long-term culture or incubation under CO₂ conditions; it is specifically designed for handling oocytes in ambient conditions.