ANDROVISION® SOFTWARE MODULE: ACROSOME INTEGRITY
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ANDROVISION® SOFTWARE MODULE: ACROSOME INTEGRITY
The AndroVision® software module: Acrosome Integrity offers a robust and automated solution for assessing the acrosome integrity of sperm across all animal species. This module seamlessly integrates with the computer-based AndroVision® semen analysis system and uses a dual-staining method based on fluorescence to distinguish between intact and damaged acrosomes.
Automated measurement of the percentage of sperm with intact versus damaged acrosomes
Objective and repeatable results across multiple animal species
Rapid analysis of large numbers of sperm in each field of view, enhancing productivity and efficiency
Integration with the AndroVision® system allows for comprehensive semen evaluation including motility, concentration, membrane integrity, mitochondrial activity, and DNA integrity
Software module compatible with the AndroVision® CASA system
Fluorescent dye set: Hoechst 33342 and FITC‑PNA for acrosome integrity assessment (dual-staining analysis)
Used with a fluorescence microscope and the AndroVision® system
Hoechst 33342 is applied to stain the nuclei of all sperm (blue) and FITC‑PNA to identify damaged acrosomes (green), allowing the software to calculate the ratios of intact and damaged acrosomes
A qualified veterinarian or laboratory specialist should determine the staining dosages and parameters based on the animal species, sample type, and clinical context
Software module code: 12500/1600
Dye set code: 15407/0011 (Hoechst 33342/FITC‑PNA)
The AndroVision® software module: Acrosome Integrity offers a robust and automated solution for assessing the acrosome integrity of sperm across all animal species. This module seamlessly integrates with the computer-based AndroVision® semen analysis system and uses a dual-staining method based on fluorescence to distinguish between intact and damaged acrosomes.
Main Benefits
Automated measurement of the percentage of sperm with intact versus damaged acrosomes
Objective and repeatable results across multiple animal species
Rapid analysis of large numbers of sperm in each field of view, enhancing productivity and efficiency
Integration with the AndroVision® system allows for comprehensive semen evaluation including motility, concentration, membrane integrity, mitochondrial activity, and DNA integrity
Composition
Software module compatible with the AndroVision® CASA system
Fluorescent dye set: Hoechst 33342 and FITC‑PNA for acrosome integrity assessment (dual-staining analysis)
Instructions for Use
Used with a fluorescence microscope and the AndroVision® system
Hoechst 33342 is applied to stain the nuclei of all sperm (blue) and FITC‑PNA to identify damaged acrosomes (green), allowing the software to calculate the ratios of intact and damaged acrosomes
A qualified veterinarian or laboratory specialist should determine the staining dosages and parameters based on the animal species, sample type, and clinical context
Package Size
Software module code: 12500/1600
Dye set code: 15407/0011 (Hoechst 33342/FITC‑PNA)