STERIDYL CSS Two-Stage, Fraction II, 500 ml Concentrate This product is a concentrated sperm extender designed for use in a two-step protocol for freezing ruminant mammal sperm. It is used by mixing it with water to prepare a ready-to-use extender medium for sperm processing.

Main Benefits

Provides a sterile and uniform extender medium for sperm freezing
Contains buffering ingredients and egg yolk to support sperm viability
Facilitates consistently high-quality freezing results across various dilution ratios
Simplifies preparation by only requiring the addition of distilled water
Enhances biosecurity using sterile ingredients and is antibiotic-free in the CSS version

Composition

TRIS buffer system (Tris-hydroxymethyl-aminomethane)
Citric acid
Sugars (energy sources)
Buffers to maintain appropriate pH
Glycerol (cryoprotectant)
Pure sterile water
Sterilized and irradiated egg yolk (provides membrane protection during freezing)

Usage Instructions

This is the second part (Fraction II) of a two-step extender and must be used within a two-part protocol. It should be mixed with the first part (Fraction I) and diluted with distilled water to achieve the final medium volume. The dilution ratio and precise preparation steps should be determined by a veterinary specialist or reproduction expert based on the type of sperm, its quality, and the processing protocol.

Package Size

500 ml of concentrated Fraction II, used to prepare approximately 1,000 ml of medium when mixed with Fraction I and water.

Note

The CSS version is antibiotic-free, enhancing biosecurity and reducing contamination risk. It is essential to follow an approved two-step protocol and ensure sterile handling during preparation.